A total of 132 White rot fungus were isolated from the wood bark samples, collected from the different locations in the Western Ghats region of Karnataka and screened for Ligninolytic enzyme production. Ten strains exhibiting maximum activity numbered 30, 40, 41, 61, 76, 86, 104, 113, 123 and 124 were further screened under submerged fermentation using agricultural wastes for enzyme production activity. Among the four substrates used (Sawdust, Paddy straw, Pongamia leaf, Kitchen Vegetable waste), highest lignin peroxidase activity was seen in Pongamia with strain 75 (0.766 U/ml) and minimum was in Paddy straw with strain 124(0.003 U/ml). Highest Manganese Peroxide content was seen in sawdust with strain 40 (1.014 U/ml) and minimum was in vegetable waste strain 75 (0.150 U/ml). Highest laccase activity was in Pongamia leafwith strain 123 (0.375 U/ml) and lowest was in Paddy straw strain (75)(0.012 U/ml).Highest versatile peroxidase activity was observed in Pongamia strain 113(0.504 U/ml) and lowest in Paddy straw strain 61 (0.023 U/ml). Cellulase content was highest with Pongamia using strain 41 (0.032 U/ml)and lowest in Paddy straw with strains61, 104, 113, 124 (0.003 U/ml).Highest Protein content was present in Pongamia degradation with strain 104(0.246 U/ml) and lowest was in Paddy straw with strain 113 (0.059 U/ml).The maximum enzyme activity of laccase by wood rot fungal strains 30, 40, 41, 61, 76, 86, 104, 113, 123 and 124 strains were obtained on the 8th day of fermentation wherein Manganese peroxidase and lignin peroxidase showed the highest activity on the 10th day of fermentation.
Volume 12 | 07-Special Issue
Pages: 1562-1569
DOI: 10.5373/JARDCS/V12SP7/20202259